Factors influencing the antioxidant effect of phospholipase A2 against lipid oxidation promoted by trout hemoglobin and hemin in washed muscle

The antioxidant effect of porcine pancreatic phospholipase A2 (PLA2) was previously demonstrated. Understanding how PLA2 inhibits lipid oxidation promoted by hemoglobin (Hb) is important for its applications in muscle foods. Effects enzyme dose, pH, and calcium ion on the ability to inhibit trout hemoglobin-mediated were investigated washed cod (WCM). Results indicated that required both hydrolyzing activity effect. abilities suppress Hb form methemoglobin ferryl pH-dependent. lag phase before enters exponential reciprocally shortened as more hemin bound insoluble matrix WCM. However, able without preventing interaction between muscle.